Welcome to PAM’s documentation!¶
PAM is short for PIE Analysis with MATLAB - it is a GUI-based software package for the analysis of fluorescence experiments and supports a large number of analysis methods. While originally designed for pulsed-interleaved excitation (PIE, [Müller2005]) experiments, it now has a wide support for the most commonly used fluorescence experiments.
Currently implemented:
- Fluorescence correlation spectroscopy and its derivatives
- FCS/FCCS/FLCS/fFCS
- Single-molecule FRET spectroscopy (burst analysis), including:
- Photon distribution analysis for two- and three-color FRET experiments
- species-selective filtered fluorescence correlation spectroscopy
- Image correlation spectroscopy, including:
- ICS/RICS/TICS/STICS
- ratser lifetime image correlation spectroscopy (RLICS)
- support for arbitrary region image correlation spectroscopy (ARICS)
- Fluorescence lifetime imaging (PhasorFLIM)
- TCSPC and time-resolved anisotropy analysis
[Lamb2000] | Lamb, D. C., Schenk, A., Röcker, C., Scalfi-Happ, C. & Nienhaus, G. U. Sensitivity Enhancement in Fluorescence Correlation Spectroscopy of Multiple Species Using Time-Gated Detection. Biophys J 79, 1129–1138 (2000) |
[Müller2005] | Müller, B. K., Zaychikov, E., Bräuchle, C. & Lamb, D. C. Pulsed interleaved excitation. Biophys J 89, 3508–3522 (2005) |
[Kudryavtsev2012] | Kudryavtsev, V. et al. Combining MFD and PIE for Accurate Single-Pair Förster Resonance Energy Transfer Measurements. ChemPhysChem 13, 1060–1078 (2012) |
[Hendrix2013] | Hendrix, J., Schrimpf, W., Höller, M. & Lamb, D. C. Pulsed Interleaved Excitation Fluctuation Imaging. Biophys J 105, 848–861 (2013) |
[Hendrix2016] | Hendrix, J., Dekens, T., Schrimpf, W. & Lamb, D. C. Arbitrary-Region Raster Image Correlation Spectroscopy. Biophys J 111, 1785–1796 (2016) |